This study was conducted on 27 normal Japanese quail embryos from the second to the 17th embryonic day (ED). The present work aimed to investigate the histological changes in the spinal cord during development and identify the immunohistochemical distribution of telocytes (TCs) and microglia. Our study revealed in the early stages (2–3 ED): development of tubular neuroepithelium and onset of neurogenesis through radial cell migration. Mid stages (5–7 ED): significant cellular remodeling and differentiation of the ventral canal. By 9–11 ED: notable structural development with expansion of gray and white matter and increased glial cell density. Advanced stages (13–15 ED): complete formation of the meninges (pia, arachnoid, dura matter). Immunohistochemically, CD34-positive TCs were detected in the pia matter (PM), white matter, and gray matter of the spinal cord, with a highly significant increase in …