Remineralisation Effect of Nano-Hydroxyapatite and Fluoride Containing Toothpastes on Artificial Enamel Caries (An In vitro Study)
ABSTRACT
Aim: This investigation was carried out to assess and evaluate the remineralization ability of
artificial enamel carious lesions by (n-HAP) tooth paste in comparison with (F) tooth paste.
Material and Methods: sixty healthy extracted teeth, the roots were cut and sectioning
crowns. The samples were placed in acrylic resin blocks, covered with nail varnish except a 3x3
mm of exposed enamel. The samples were divided into three groups based on the toothpaste
used: GA1 (n-hap), GA2 (F) and GA3 (n-hap+ F). The initial enamel lesion was produced using
a demineralizing solution. Applying toothpaste for 30 cycles twice daily, and washing them under
running water. In pH-cycling models, samples were demineralized twice daily for three hours,
followed by a two-minute treatment with a remineralizing agent slurry twice daily over a 24-hour
cycle. After two weeks and one month of brushing, specimens were examined utilizing (EDAX)
and (SEM).
Results: At baseline and after demineralization, there was no statistically significant difference
found between the three tested toothpastes, respectively, while after 2 weeks, the statistical analysis
showed G A1 (n-hap) was significantly higher than of GA2 (F) and GA3 (n-hap+ F). After 1 month,
there was a statistically significant difference as G A1 (n-hap) was significantly higher than GA2
(F) and GA3 (n-hap+ F).
Conclusions: Nanohydroxyapatite-containing toothpaste exhibits superior remineralizing
potential as compared to fluoride-containing toothpaste
